Journal: Heliyon

Article Title: Coordination of early cellular reactions during activation of bone resorption in the rat mandible periosteum: An immunohistochemical study

doi: 10.1016/j.heliyon.2017.e00430

Figure Lengend Snippet: Osteogenic compartment. a and b : Immunostaining for RANKL. At baseline (Bs) a few immunopositive cells are scattered within the compartment. At 18 h ( b ), RANKL+ cells were more numerous. The staining was stronger than at baseline. Bar = 50 μm. c Changes with time in RANKL+ cells. d Variations in osteogenic cells and osteocytes expressing semaphorin3a. e and f Immunostaining for semaphorin 3a. At baseline most osteogenic cells were immunopositive as were the osteocytes (arrows) subjacent to the bone surface. At 24 h, only a few cells expressed the marker while the osteocytes no longer expressed it. Bar = 50 μm. * p ≤ 0.05; ** p<0.01; *** p<0.005 versus baseline. ° p<0.02; °° p<0.003 versus 18 h. # p<0.05 versus 24 h.

Article Snippet: Mouse monoclonal antibodies against HIS48 (ref 554905, BD Biosciences Pharmingen, San Diego, CA, USA: 1:100), ED1 (murine equivalent of CD68, MAB1435, Chemicon, Temecula, CA, USA; 1:100), CD54 (ICAM-1, ref 554967, BD Biosciences Pharmingen, 1:50) and rabbit polyclonal antibodies against CD106 (VCAM-1, SC-8304, Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:50), RANKL (ref 3963, ProSci, Poway, CA, USA; 1/160), semaphorin 3a (ab23393, Abcam, Cambridge, UK; 1:50), laminin (L9393, Sigma; 1:25) were used.

Techniques: Immunostaining, Staining, Expressing, Marker

Journal: Medicine

Article Title: Determination of diagnostic and predictive parameters for vertical mandibular invasion in patients with lower gingival squamous cell carcinoma: A retrospective study

doi: 10.1097/MD.0000000000032206

Figure Lengend Snippet: Immunohistochemical staining.

Article Snippet: IL-6 , Rockland Inc. , 1:2000 , Rabbit polyclonal antibody , • Inflammatory cytokines released by cancer cells. • Since stimulation by IL-6 and TNF-α induces bone-resorbing multinucleated giant cells, it is possible that some mechanisms of the bone-resorptive effects of IL-6 are not mediated by the RANK/RANKL pathway..

Techniques: Immunohistochemical staining, Staining, Binding Assay, Activity Assay, Expressing

Journal: Molecular Medicine Reports

Article Title: Effectiveness of combined salmon calcitonin and aspirin therapy for osteoporosis in ovariectomized rats

doi: 10.3892/mmr.2015.3637

Figure Lengend Snippet: Combined treatment (sCT+ASA) enhances the OPG/RANKL expression ratio in OVX rats. Effects of ASA, sCT and combined treatment (sCT+ASA) on mRNA levels in femur bone marrow cells from ovariectomized (OVX) rats. Gene expression was analyzed by reverse transcription-quantitative polymerase chain reaction. Values are expressed as the mean ± standard deviation. P-values were calculated by analysis of variance with Student-Newman-Keuls analysis. (A) OPG; (B) RANKL; (C) mRNA expression ratio of OPG/RANKL. ASA, aspirin; sCT, salmon calcitonin; OVX, ovariectomized; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor κB ligand; mRNA, messenger RNA.

Article Snippet: The antibodies used were as follows: OPG primary antibody (1:80; BA1475; Wuhan Boster Biological Technology Co. Ltd., Wuhan, China; rabbit anti-rat OPG), RANKL primary antibody (1:80; BA1323; Wuhan Boster Biological Technology Co. Ltd.; rabbit anti-rat OPG ligand) and rabbit anti-rat biotinylated secondary antibody (SP-0023; Beijing Biosynthesis Biotechnology Co., Ltd., Beijing, China).

Techniques: Expressing, Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Effectiveness of combined salmon calcitonin and aspirin therapy for osteoporosis in ovariectomized rats

doi: 10.3892/mmr.2015.3637

Figure Lengend Snippet: Combined treatment significantly enhances the OPG/RANKL ratio in the proximal tibia of OVX rats. Effects of ASA, sCT and combined treatment (sCT+ASA) on protein expression levels in the proximal tibia of OVX rats. Specimens were observed at magnification, x250. Antigens were detected using an avidin-biotin-peroxidase system and visualized with 3,3′-diaminobenzidine. Protein levels were measured by densitometry. Values are expressed as the mean ± standard deviation. P-values were calculated by analysis of variance, with Student-Newman-Keuls analysis). (A) OPG; (B) RANKL; (C) OPG/RANKL protein ratio. Positive staining for OPG and RANKL was mainly localized in the chondrocytes of the epiphyseal growth plate and trabeculae of bone. ASA, aspirin; sCT, salmon calcitonin; OVX, ovariectomized; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor κB ligand.

Article Snippet: The antibodies used were as follows: OPG primary antibody (1:80; BA1475; Wuhan Boster Biological Technology Co. Ltd., Wuhan, China; rabbit anti-rat OPG), RANKL primary antibody (1:80; BA1323; Wuhan Boster Biological Technology Co. Ltd.; rabbit anti-rat OPG ligand) and rabbit anti-rat biotinylated secondary antibody (SP-0023; Beijing Biosynthesis Biotechnology Co., Ltd., Beijing, China).

Techniques: Expressing, Avidin-Biotin Assay, Standard Deviation, Staining